Chromosome-Based Genetic Complementation System for Xylella fastidiosa
نویسندگان
چکیده
منابع مشابه
Chromosome-based genetic complementation system for Xylella fastidiosa.
Xylella fastidiosa is a xylem-limited, gram-negative bacterium that causes Pierce's disease of grapevine. Here, we describe the construction of four vectors that facilitate the insertion of genes into a neutral site (NS1) in the X. fastidiosa chromosome. These vectors carry a colE1-like (pMB1) replicon and DNA sequences from NS1 flanking a multiple-cloning site and a resistance marker for one o...
متن کاملMapping analysis of the Xylella fastidiosa genome.
A cosmid library was made of the 2.7 Mb genome of the Gram-negative plant pathogenic bacterium Xylella fastidiosa and analysed by hybridisation mapping. Clones taken from the library as well as genomic restriction fragments of rarely cutting enzymes were used as probes. The latter served as a backbone for ordering the initial map contigs and thus facilitated gap closure. Also, the co-linearity ...
متن کاملInteraction between Xylella Fastidiosa and Curtobacterium Flaccumfaciens
Xylella fastidiosa (Xf) is a fastidious gram-negative, xylem-limited bacterium that causes diseases in many crops of economic importance, such as grape (Pierce’s disease), almond, peach, coffee, plum and citrus. Xf can infect all known Citrus sinensis cultivars and causes Citrus variegated chlorosis (CVC). One major endophyte isolated from CVC-asymptomatic plants is the bacterium Curtobacterium...
متن کاملMultilocus simple sequence repeat markers for differentiating strains and evaluating genetic diversity of Xylella fastidiosa.
A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR primer design and were validated in PCR experiments. These multilocus SSR primers, ...
متن کاملExploiting Xylella Fastidiosa Proteins for Pierce ’ S Disease Control
The aim of this project is to construct and express in grapevine, a protein or protein chimera capable of inactivating or otherwise interfering with the infectivity of, or disease-induction by, Xylella fastidiosa (Xf), the causative agent of Pierce’s disease (PD) of grapevine. A single-chain, monoclonal (scFv) antibody was selected for its ability to bind to Xf cells recovered by centrifugation...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2009
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.00024-09